The enzyme production profile before optimization indicated. A total of 31 molds were isolated and thereafter screened for their ability to produce xylanase by. The aim of this work is to enhance xylanase production using aspergillus niger by optimization of. Xylanase production by indigenous aspergillus candidus using wheat bran as a substrate under solid state fermentation was studied. The production of xylanase by an isolated strain of aspergillus niger in solid state fermentation was investigated. This technology can be used with a wide range of microorganisms. Xylanase production by a new bacillus pumilus sy30a. Pdf xylanase and pectinase production by aspergillus. The use of surfactants such as triton x100, tween80 during smf or ssf was found to increase the enzyme production 16 17 18. Xylanase production under solid state and submerged. Optimization of xylanase production by thermomyces.
The present study showed that solid substrate to fermentation medium ratio of 1. The order of xylanase production was as follows, a. To optimize the cultivation conditions for xylanase production by solid state fermentation ssf using bacillus pumilus, with paddy husk as support, solid medium contained 200 g of paddy husk with 800 ml of liquid fermentation medium gl xylan, 20. Xylanase production by trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse. Pdf the production of xylanase by an isolated strain of aspergillus niger in solid state fermentation was investigated. Ssf 2 was performed using wheat bran, sorghum stover, corn cob and soybean meal in flasks for. Streptomyces thermocerradoensis i3 secretes a novel. It was obvious to see that the production of xylanase was increased by the increase of ph from 3. Xylanase production by aspergillus awamori under solid state fermentation conditions on tomato pomace. Pdf production and optimization of xylanase from penicillium. Pdf xylanase production by trichoderma harzianum rifai. The ssf is alternative to submerged fermentation for production of value added products like. In the present study, xylanase production by 21 fusarium sp. Purification and characterization of xylanase srxl1.
Prior to the current study, our understanding of genomewide gene expression and of the regulation of gene expression of lignocellulosedegrading enzymes in ascomycete fungi during ssf was. Improvement of cellulase and xylanase production by solid. Contribution of proteases and cellulases produced by solid. The extracellular xylanase was isolated by ammonium sulfate 80% precipitation and purified using ion exchange chromatography. Optimization of process parameters for xylanase production. Xylanase production from aspergillus niger by solid state. Development and fabrication of solid state fermenter in this study, a laboratory scale solid state fermenter namely fermsostat was developed by the authors and fabricated by a local fabricator. Growth and xylanase production the fungus did not grow in xam and xm media. Production of cellulase and xylanase using food waste by. Among lignocellulosic substrates tested wheat straw, sugarcane baggase, rice. Extracellular xylanase production by fusarium species in. Yeasts have not been reported to utilize sb, particularly under solidstate fermentation ssf. The data presented in this paper show the possibility of the use of agricultural wastes, corn cob, sugar cane bagasse and wheat bran to produce xylanolytic enzymes in solid state fermentation by a.
High xylanase activity 1079 ug was obtained when wheat straw was used after 8 days of incubation. The wet substrate in the flask was autoclaved at 121. During solid state fermentation, various carbon and nitrogen sources in place of wheat bran viz. Xylanase production using alkalothermophilic bacillus. An investigation was carried out to study the production and optimal activity of exo xylanase by solid state fermentation using sugar cane bagasse as the agricultural residue. A thermoalkalophilic new species of bacillus, similar to bacillus arseniciselenatis dsm 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. Xylanase production by aspergillus awamori under solid. Under these optimi zed conditions, a xylanase activity of 3099 iug of dry matter was obtained. Solidstate fermentation for xylanase production by. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production 1543 and 1483 ug. Paddy husk as support for solid state fermentation to.
Extracellular xylanase production by the thermophilic fungus thermomyces lanuginosus 195 in solid state fermentation ssf was found to be signi. Optimisation of solidstate fermentation of aspergillus niger jl15. Xylanase production under solid state fermentation the strains were further tested for their abilities to produce extracellular xylanase under solid state fermentation. Xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. In gym, there was no activity during the first 48 h of. Delay in the enzyme production in solid state fermentation could be due to the accumulation of nutrients within the. Xylanase production from bacillus pumilus vlk1 in solid state fermentation was enhanced through optimization of the process using one variable approach. The developed solid state fermenter was used throughout the study of ssf process for enzyme production.
We investigated xylanase production by thermoascus aurantiacus using semisolid fermentation. Xylanase production by aspergillus awamori under solid state. Two stage statistical designs were used to optimize xylanase production from a newly isolate bacillus licheniformis under solid state fermentation. Xylanase production by different strains in solid state fermentation ssf.
Optimization of these parameters corresponded to a 21. This study aims to optimize the medium for xylanase activity by a newly isolated strain of rhizopus stolonifer js1008 r. The growing interest in using solid state fermentation ssf techniques to produce a wide variety of enzymes, including xylanases from fungal origins, is primarily due to the economic and. Xylosidase enzymes by pseudozyma hubeiensisin solid state fermentation. An aliquot of the broth was taken out after every 24. Solid state fermentation, submerged fermentation 1. Xylanases encompass a broad spectrum of industrial applications like food, animal feed, textile and biofuel.
The initial moisture content, cultivation time, inoculum size and concentration of basal medium were optimized in solid state fermentation ssf for the production of xylanase by an aspergillus niger mutant using statistical experimental designs. The initial moisture content and bagasse mass were the most important factors affecting. Solidstate fermentation ssf is defined as the growth of microbes without free flowing aqueous phase. Comprehensive studies on optimization of cellulase and. Optimization of nutrition constituents for xylanase. Fermentation was carried out with trichoderma koeningi isolate using untreated and pretreated corn cob supplemented with pineapple peel powder showed higher. Solidstate fermentation ssf on five agroindustrial wastes showed differential utilization of the various carbon sources by the three isolates. The main objective of this study was to isolate the fungal strain for enhanced production of xylanase using different agroresidues and fruit peels by solid state fermentation and its potentiality was tested on the pretreated corn cob. Statistical optimization of medium composition for. Xylanase and pectinase production by aspergillus awamori on grape pomace in solid state fermentation.
Production of xylanases from fungal isolates by solid. Effect of initial medium ph for xylanase production. The present study investigated the production of xylanases and cellulases by streptomyces thermocerradoensis i3 under solid. Xylanase production in solid state fermentation by. Solidstate fermentation 114 ssf process was conducted in the 500 ml erlenmeyer flask and each flask contained 20 g of food waste 115 wet weight. The use of abundantly available and costeffective agricultural residues, such as wheat bran, corn cobs, rice bran, rice. Solidstate fermentation ssf, that simulates the natural habitat of soil microorganisms, is used for a variety of applications such as biomass biorefinery. Solidstate fermentation processes are practical for complex substrates, including agricultural, forestry and food processing residues and wastes, which are used as inducing carbon sources for the production of xylanases.
Bioprocessing of agroindustrial residues for optimization. Xylanase production by ganoderma lucidum on liquid and. The cultivation time and concentration of basal medium were the most important factors affecting xylanase activity. Production and optimization of xylanase from penicillium species in solidstate fermentation. Figure 1a shows the xylanase activity produced by smf. The purpose of this work was to study the production of the endoglucanase cmcase carboxymethyl cellulase and xylanase of an isolated brazilian strain of thermoascus. Purification and characterization of xylanase the purification factor of xylanase srxl1 was 36fold table 1. The use of solidstate fermentation technology to produce industrial enzymes is common in eastern countries such as india, china or japan, and has been of growing interest for numerous applications worldwide over the past few decades bhargav et al. The purpose of this work was to produce xylanase from arthrobacter sp mtcc 6915 under solidstate fermentation using saw dust as substrate and also to study the influence of conditions of fermentation in enhancing the production of the enzyme and the effect of metal ion concentration on xylanase and also to determine the effect of ph and.
Madurai kamaraj university, madurai 625 021, india. Multivariant statistical approaches were employed to evaluate the effects of several variables initial moisture in the medium, cultivation time, inoculum level, and bagasse mass on xylanase production. The high cost of cellulases remains the most significant barrier to the economical production of bio. The goal of this study was to optimize cellulases and xylanase production by a local indigenous fungus strain aspergillus niger dwa8 using agricultural waste oil palm frond opf as substrate. Serial no name of strain xylanase iu gds1 cellulase iu gds1 protein mg gds1 1 aspergillus niger sbs3 724 10 18 2 a. Optimization of nutrition constituents for xylanase activity by rhizopus stolonifer under solidstate fermentation on corncob zhicai zhang, a,c, jiashao li, a fan feng, b dan liu, a qiaoxia pang, a ming li, a and keping chen b this study aims to optimize the medium for xylanase activity by a newly isolated strain of rhizopus stolonifer js1008 r.
Four quantitative variables impacting the enzyme activity were selected through onefactoratatime design. Optimization of xylanase production by filamentous fungi in solidstate fermentation and scaleup to horizontal tube bioreactor n. Xylanase production by aspergillus niger lpb 326 in solidstate. Production of xylanolytic enzymes by aspergillus tamarii. Solid state fermentation was carried out upto 120 hours. Production of an alkali tolerant extracellular xylanase. For each fungal strain, two replicates of the congo red 112 test were performed. Efficiency of developed solid state bioreactor fermsostat. Xylanase production by aspergillus niger via solid state fermentation was carried. Plackett burmann and central composite design in response surface methodology were used to build statistical models to screen out the significant variables and then study the effect of three significant variables on xylanase production.
Xylanase production by aspergillus niger van tieghem was studied in solidstate cultivation. Introduction lignocellulosic biomass, being renewable, is the only sustainable source of orhow to cite this paper. A comparison of both activities was first performed in submerged cultures using various concentrations of wb, glucose, and cellulose as substrates. Xylanase production by streptomyces viridosporus t7a in submerged and solid state fermentation using agroindustrial residues. Fungal xylanase production under solid state and submerged.
Microbial strategies for cellulase and xylanase production. Improvement of xylanase production by penicillium canescens 1010c in solidstate fermentation allah antoine assamoi, franck delvigne, jeanmarc aldric, jacqueline destain, philippe thonart gembloux agricultural university fusagx. The current study investigated the production of cellulases and xylanases from the rare fungus stachybotrys microspora under solid. Sepahy, ghazi, and sepahy 2011 reported fermentation period of 48 h by bacillus mojavensis ag7 in submerge fermentation using oat bran as substrate. For this, the strains were cultured in erlenmeyer flasks 250 ml containing 10 g of wheat bran. Ultrasounds pretreatment of olive pomace to improve. Inorganic nitrogen source ammonium nitrate was found best among. The screening of substrates was carried out in column incubato. There are very few reports on the production of cellulase free xylanases especially by yeast strains which have great potential in paper and pulp industry in removing the hemicellulose from the treated or untreated pulp. In this work, two yeast strains, mk157 and mk118 were cocultured to produce a multienzyme preparation comprised of endoglucanase eg. The xylanase production by cochliobolus sativus strain cs6 was improved under solid state fermentation ssf. The production of xylanase xyla by aspergillus niger jl15 in solidstate fermentation ssf on orange peel was optimised by the response surface. Optimization of xylanase production by filamentous fungi.
Pdf xylanase production by aspergillus awamori under. This study aimed at optimization of solidstate fermentation by aspergillus niger ccug33991 for maximum xylanase production using lowcost agroindustrial residues 1 in tray bioreactor. The activities of xylanase and carboxymethyl cellulase cmcase were evaluated until 96 hr of incubation. Improved xylanase production by mixing low cost wastes and.
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